| 摘要 |
The present invention relates to a surface-modified biocompatible silk fibroin film, and to a method for producing the same. More specifically, the present invention relates to a biocompatible silk fibroin film which is very useful in producing a tissue-engineering artificial bio-corneal endothelial layer for transplantation, which can replace donated cornea lacking in the future. According to the present invention, a film is produced by using a silk fibroin (or a silk protein), and the properties of the surface thereof are enhanced through surface modification, so cell adhering and proliferating effects are improved. When a tissue-engineering bio-corneal endothelial layer which is suitable for bio-transplantation is produced and applied by using the surface-modified film, the tissue-engineering bio-corneal endothelial layer has maintained transparency, and cultured cells constitute a single layer. Moreover, the number of corneal endothelial cells needed for transplantation is sufficient, so functions of the corneal endothelial cells can be excellently exhibited after being cultured. |
