发明名称 |
METHOD OF PRODUCING RECOMBINANT HIGH MOLECULAR WEIGHT VWF IN CELL CULTURE |
摘要 |
Among other aspects, the present invention relates to cell culture conditions for producing high molecular weight vWF, in particular, highly multimericWF with a high specific activity and ADAMTS 13 with a high specific activity. The cell culture conditions of the present invention can include, for example, a cell culture medium with an increased copper concentration and/or cell culture supernatant with a low ammonium (NH 4 + ) concentration. The present invention also provides methods for cultivating cells in the cell culture conditions to express high molecular weight vWF and rA13 having high specific activities. |
申请公布号 |
EP3064508(A1) |
申请公布日期 |
2016.09.07 |
申请号 |
EP20160153131 |
申请日期 |
2011.07.08 |
申请人 |
BAXALTA GMBH;BAXALTA INCORPORATED |
发明人 |
GRILLBERGER, LEOPOLD;REITER, MANFRED;MUNDT, WOLFGANG |
分类号 |
C07K14/755;A61K38/00;A61K38/36;A61K39/00;C12N5/00;C12N5/10;C12N9/64 |
主分类号 |
C07K14/755 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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