SEPARATION AND PURIFICATION METHOD FOR VANCOMYCIN HYDROCHLORIDE OF HIGH PURITY
摘要
Provided is a separation and purification method for vancomycin hydrochloride of high purity. The method comprises the following steps: (1) obtaining a vancomycin hydrochloride solution from a crude vancomycin product by ion exchange chromatography and obtaining a concentrate by nanofiltration desalination and concentration; (2) adjusting the concentrate with a hydrochloric acid solution and then performing a column chromatography using a reverse chromatography column for the adjusted concentrate; (3) collecting the chromatographic solution of vancomycin to obtain a mixed chromatographic solution; (4) adjusting the mixed chromatographic solution, and separating the solution and the salts by nanofiltration desalination and concentration to obtain a concentrate; and (5) obtaining a vancomycin dry powder with a chromatographic purity of up to 99% and a pure white appearance by dehydrating and drying the concentrate of step (4), or by solvent crystallization or salting-out crystallization.
申请公布号
EP3064214(A1)
申请公布日期
2016.09.07
申请号
EP20140857463
申请日期
2014.10.27
申请人
ZHEJIANG MEDICINE CO., LTD. XINCHANG PHARMACEUTICAL FACTORY