DAB-CONTAINING SUBSTRATE KIT FOR DYEING USE WHICH IS PRODUCED USING LABELLING ENZYME

摘要

Provided is a DAB-containing substrate kit that is capable of enhancing specific staining intensity and reducing precipitation due to DAB aggregation in DAB-containing substrate solution and nonspecific staining, such as background staining, in chromogenic reaction using a peroxidase-labeled antibody, by the use of chromogenic reaction solution containing DAB and its sensitizer imidazole. The kit includes chromophore-containing solution (A) containing a peroxidase chromophore DAB and water; and chromogenic reagent (B) containing: at least one chelating agent selected from the group consisting of EDTA-2Na, EDTA-2NH 4 , GEDTA, and NTA; POE and/or POP alkyl ether nonionic surfactant; imidazole; hydrogen peroxide; and water. The kit is in a system of two- or more components with at least the chromophore-containing solution (A) and the chromogenic reagent (B) being separately stored.