New gene products of bacillus licheniformis forming or decomposing polyamino acids and associated improved biotechnological production method

摘要

Proteins (I) designated YwsC (CapB; PgsB); YwsC' (a short version of YwsC); YwtA (CapC; PgsC); YwtB (CapA; PsgA) and YwtD (Dep; PgdS) are new. They are involved in formation of polyamino acid (PAA), except for YwtD which breaks down PAA. Independent claims are also included for: (i) nucleic acid (II) that encodes (I), designated ywsC; ywsC'; ywtA; ywtB and ywtD; (ii) vector that contains (II); (iii) cells genetically modified to contain (II); (iv) preparation of (I); (v) microorganisms in which at least one of ywsC, ywsC', ywtA or ywtB is functionally inactivated or ywtD is overactive; (vi) method for fermentation of (A); and (vii) microbial or cell-free process for preparation, modification or degradation of poly(g-glutamate) (PGG).