发明名称 多能性幹細胞の選別方法
摘要 The present invention provides a novel method for evaluating/selecting a pluripotent stem cell with high efficiency and high accuracy while eliminating the occurrence of a cross reaction as observed in an antigen-antibody reaction. In the present invention, an N-binding sugar chain, an O-binding sugar chain, a GSL, a GAG, and a FOS on the surface of a cell are separated and purified from a single cell sample by a glycoblotting method or a BEP method, and the comprehensive quantitative/qualitative analysis of these sugar chains is carried out by MALDI-TOF/MS. In this manner, sugar chain markers specific to a pluripotent stem cell can be determined, and the sugar chain markers can be used for the identification of a pluripotent cell.
申请公布号 JP5979452(B2) 申请公布日期 2016.08.24
申请号 JP20140521190 申请日期 2012.11.05
申请人 国立大学法人北海道大学 发明人 篠原 康郎;古川 潤一;藤谷 直樹;荒木 香代;中村 幸夫
分类号 C12Q1/04;G01N27/62;G01N33/48;G01N33/53 主分类号 C12Q1/04
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