发明名称 |
PRODUCTION OF ANTI-AMYLOID BETA ANTIBODIES |
摘要 |
An improved system for large scale production of proteins and/or polypeptides in cell culture, particularly in media characterized by one or more of i) a cumulative amino acid concentration greater than about 70 mM; ii) a molar cumulative glutamine to cumulative asparagine ratio of less than about 2; iii) a molar cumulative glutamine to cumulative total amino acid ratio of less than about 0.2; iv) a molar cumulative inorganic ion to cumulative total amino acid ratio between about 0.4 to 1; or v) a combined cumulative glutamine and cumulative asparagine concentration between about 16 and 36 mM, is provided. The use of such a system allows high levels of protein production and lessens accumulation of certain undesirable factors such as ammonium and/or lactate. Additionally, culture methods including a temperature shift typically including a decrease in temperature when the culture has reached about 20-80% of it maximal cell density, are provided. Alternatively or additionally, the present invention provides methods such that, after reaching a peak, lactate and/or ammonium levels in the culture decrease over time. |
申请公布号 |
EP1781803(B1) |
申请公布日期 |
2016.08.24 |
申请号 |
EP20050791655 |
申请日期 |
2005.08.26 |
申请人 |
PFIZER IRELAND PHARMACEUTICALS |
发明人 |
DRAPEAU, DENIS;LUAN, YEN-TUNG;MERCER, JAMES, R.;WANG, WENGE;LASKO, DANIEL R. |
分类号 |
C07K16/18;C07K16/00;C07K16/22;C07K16/28;C12N5/00;C12P21/00;C12P21/08 |
主分类号 |
C07K16/18 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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