摘要 |
A pipette tip 16 comprising a vessel prefilled with a reagent 36 to permit an assay to be performed in the tip, and a pipette 12 comprising a body portion 14, for aspirating a fluid into the pipette tip when attached, with at least one light source 26, or at least one entry point for a light source, providing an optical path of light that passes through the sample in a direction along the longitudinal axis of the pipette tip. A method for measuring light output from a fluid sample in a pipette tip or tube having first and second open ends 46, 68 (figure 2), by detecting light output from an open end of the tip or tube, wherein the sample is drawn into the pipette tip in a number of steps, and shining a light longitudinally through the tip during the step process to obtain a calibration curve of light detected. A kit for light measurement comprising the prefilled pipette tip or tube, a light output guide means 66 for guiding light output from an open end of the pipette tip or capillary tube to a photodetector 48 (figure 4). |