发明名称 |
UBIQUITINYLATED PROTEINS |
摘要 |
A method of inducing a specific immune response in a mammal, comprising: providing a first composition comprising isolated ubiquitinylated proteins in solution in the absence of membrane bound organelles, the isolated ubiquitinylated proteins comprising one or more specific antigens, and further comprising a threshold quantity of polyubiquitinylated short-lived proteins and polyubiquitinylated defective ribosomal products. The isolated ubiquitinylated proteins are affinity-purified from tumor-derived cells grown in culture, the tumor-derived cells being inhibited from degrading ubiquitinylated proteins via the proteasome while being grown in culture. In this way, highly immunogenic short-lived proteins and defective ribosomal products may be loaded onto dendritic cells for cross-presentation and priming of antigen-specific T cells restricted by either classical or non-classical MHC. |
申请公布号 |
EP3055322(A1) |
申请公布日期 |
2016.08.17 |
申请号 |
EP20140851719 |
申请日期 |
2014.10.10 |
申请人 |
UBIVAC, LLC;PROVIDENCE HEALTH & SERVICES - OREGON |
发明人 |
TWITTY, CHRISTOPHER;LESHANE, ERIK;FOX, BERNARD;HU, HONG-MING;YU, GUANGJIE |
分类号 |
C07K14/435;A61K38/17;A61P37/02 |
主分类号 |
C07K14/435 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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