| 摘要 |
[Problem] The present invention provides: a novel endo-²-N-acetylglucosaminidase (Endo-Om); and a gene for the endo-²-N-acetylglucosaminidase (Endo-Om). [Solution] The present invention provides a novel endo-²-N-acetylglucosaminidase (Endo-Om) using a transformant produced by cloning an endo-²-N-acetylglucosaminidase (Endo-Om) gene originated from a methanol-utilizing yeast Ogataea minuta IFO10746 strain. The Endo-Om according to the present invention has a specific activity 13-fold higher than that of known Endo-M and a Vmax value 55-fold higher than that of the known Endo-M, and is useful for the analysis of the structures of sugar chains, including composite sugar chains, in glycoproteins and the modification of the sugar chains. Also provided are an endo-²-N-acetylglucosaminidase (Endo-Cp), an endo-²-N-acetylglucosaminidase (Endo-Pa) and an endo-²-N-acetylglucosaminidase (Endo-Zr) which are produced from Candida parapolymorpha DL-1 ATCC26012 strain, Pichia anomala ATCC36904 strain and Zygosaccharomyces rouxii ATCC2623 strain, respectively, on the basis of an Endo-Om gene sequence, and each of which has a similar level of composite sugar chain cleavage activity and a similar level of composite sugar chain transfer activity to those of Endo-Om. |
