TARGETED INTEGRATION INTO STEM CELLS

摘要

PROBLEM TO BE SOLVED: To provide methods and compositions for targeted integration of sequences of interest such as lineage-specific or cell fate reporter constructs or protein encoding sequences into stem cells.SOLUTION: A stem cell comprises a transgene, where the transgene comprises a gene product and is integrated into a specified region of the genome of the stem cell such that the gene product is transcribed or translated upon or during differentiation of the stem cell into a lineage-specific or mature cell type.SELECTED DRAWING: Figure 8