| 摘要 |
Induced pluripotent stem cells having genetic information identical to that of a patient and yet having nature close to those of ES cells are prepared from human peripheral blood monocytes without leaving those genes in the resultant cells after use for their preparation. Reprogramming genes are loaded on sustained expression-inducing Sendai viral vectors which do not have an activity for integrating foreign genetic information into chromosomes and they are introduced into peripheral blood-derived monocytes to be expressed. Subsequently, the vector genome RNA comprising the reprogramming genes is removed from the cells to establish induced pluripotent stem cells. The above-described Sendai viral vector is capable of extremely simple and efficient preparation of induced pluripotent stem cells having genetic information identical to that of an individual who supplied the differentiated cell and yet this vector is safe presenting only a low risk of tumorigenesis. By supplying useful induced pluripotent stem cells, this Sendai viral vector can serve as a powerful tool in various applications including safe and efficient performance of cell replacement therapy. |
