发明名称 |
METHODS OF MAMMALIAN RETINAL STEM CELL PRODUCTION AND APPLICATIONS |
摘要 |
The invention provides an in vitro method for producing isolated mammalian primitive retinal stem cells (pRSCs) comprising: (a) culturing isolated embryonic stem cells (ESCs) from a mammal in a cell culture medium that is free of feeder cells, feeder-conditioned medium or serum so as to produce and grow a culture of the isolated ESCs; and (b) contacting the culture of the isolated ESCs so grown with one or more of an inhibitor for Wnt or TGF-β/BMP signaling so as to differentiate the isolated ESCs of (a) into primitive retinal stem cells thereby producing isolated mammalian pRSCs. |
申请公布号 |
EP3047017(A2) |
申请公布日期 |
2016.07.27 |
申请号 |
EP20140852550 |
申请日期 |
2014.10.09 |
申请人 |
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA |
发明人 |
ZHANG, KANG;ZHAO, JIAGANG;ALMUTAIRI, ADAH |
分类号 |
C12N5/00;C12N5/02 |
主分类号 |
C12N5/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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