发明名称 |
TRANSFORMANT OF SCHIZOSACCHAROMYCES POMBE MUTANT, AND CLONING VECTOR |
摘要 |
Provided is a transformant of S. pombe mutant which can produce and collect ²- glucosidase without requiring complicated separation steps, and a vector which is useful for transforming a yeast of the genus Schizosaccharomyces. The transformant of a S. pombe mutant of the present invention is a transformant of a Schizosaccharomyces pombe mutant which exhibits an increased Gsf activity and decreased or no pyruvyl transferase Pvg1 enzymatic activity, and is comprised of a structural gene sequence encoding a ² -glucosidase derived from a filamentous fungus, and a promoter sequence and a terminator sequence for expressing the structural gene in a chromosome or as an extrachromosomal gene. Further, the present invention relates to a cloning vector which is characterized by comprising a hsp9 gene promoter or an ihc1 gene promoter of a yeast of the genus Schizosaccharomyces, and is useful for transforming a yeast of the genus Schizosaccharomyces, an expression vector, a transformant, etc. |
申请公布号 |
EP2886642(A4) |
申请公布日期 |
2016.07.27 |
申请号 |
EP20130830894 |
申请日期 |
2013.08.20 |
申请人 |
ASAHI GLASS COMPANY, LIMITED |
发明人 |
KOBAYASHI, MAYUMI;TOHDA, HIDEKI;KIMURA, SHUICHIRO;ZEN-IN, SUSUMU;HARA, FUTOSHI |
分类号 |
C12N1/19;C12N15/09 |
主分类号 |
C12N1/19 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|