发明名称 |
METHOD FOR SOLID PHASE SYNTHESIS OF LIRAGLUTIDE |
摘要 |
Provided is a method for solid phase synthesis of liraglutide, comprising the following steps: A), Fmoc-Gly-resin being obtained by coupling resin solid phase carrier with glycine with N-end protected by Fmoc(Fmoc-Gly-OH) in the presence of activator system; B) according to the peptide sequence of the main chain of liraglutide, successively coupling with amino acids with N-ends protected by Fmoc and protected side chains by the method for solid phase synthesis, wherein lysine employs Fmoc-Lys(Alloc)-OH; C) removing the protective group, Alloc, from the side chain of lysine; D) couplilng the side chain of lysine with Palmitoyl-Glu-Offiu by the method for solid phase synthesis; E) cleavage, removing protection groups and resin to obtain crude liraglutide; F) purifying and lyophilizing to obtain liraglutide. |
申请公布号 |
EP2757107(B1) |
申请公布日期 |
2016.07.27 |
申请号 |
EP20120831927 |
申请日期 |
2012.08.30 |
申请人 |
HYBIO PHARMACEUTICAL CO., LTD. |
发明人 |
PAN, JUNFENG;LIU, JIAN;MA, YAPING;YUAN, JIANCHENG |
分类号 |
C07K14/605;A61P3/10;C07K1/04;C07K1/06 |
主分类号 |
C07K14/605 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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