发明名称 内在性DNA配列特異的結合分子を用いる特定ゲノム領域の単離方法
摘要 Provided is a method comprising (A) fragmenting genomic DNA in a state where the interaction of the genomic DNA and molecules interacting therewith is maintained, and (B) bringing genomic DNA into contact with an exogenous molecule capable of binding to a specific endogenous DNA sequence in the genomic DNA. According to the method, with the use of an endogenous DNA sequence present inside or in the vicinity of a target genomic region in cells to be analyzed, any genomic region can be specifically isolated in a state where the interaction of the genomic region and molecules interacting therewith is maintained, without the need of inserting a recognition sequence of an exogenous DNA-binding molecule into the vicinity of the target genomic region in the cells to be analyzed.
申请公布号 JP5954808(B2) 申请公布日期 2016.07.20
申请号 JP20150500088 申请日期 2013.09.06
申请人 国立大学法人大阪大学 发明人 藤井 穂高;藤田 敏次
分类号 C12N15/09;C12Q1/02;C12Q1/68;G01N33/53;G01N33/543 主分类号 C12N15/09
代理机构 代理人
主权项
地址