IMMUNOGLOBULIN-BINDING DOMAIN MULTIMER

摘要

Provided herein are a multimeric immunoglobulin-binding protein having improved properties as an affinity ligand for affinity chromatography, and an insoluble support immobilizing such a multimer. The immunoglobulin-binding protein is represented by the following general formula:
€ƒ€ƒ€ƒ€ƒ€ƒ€ƒ€ƒ€ƒ(R1) n -(R2) m ,
or
€ƒ€ƒ€ƒ€ƒ€ƒ€ƒ€ƒ€ƒ(R2) m -(R1) n ,
wherein R2 is an immunoglobulin-binding domain that includes an amino acid residue that covalently bonds to an insoluble support upon immobilization reaction with the insoluble support, and R1 is an immunoglobulin-binding domain that does not contain an amino acid residue the presence of which in the sequence compared to when it is absent in the sequence reduces the immunoglobulin-binding activity of the support yielded by the immobilization reaction. The immunoglobulin-binding protein satisfies the following conditions:
(1) n is an integer of 5 to 9;
(2) m is an integer of 1 or 2;
(3) the n (R1) domains may or may not have the same sequence; and
(4) the total number of domains (n + m) is 6 to 10.