| 摘要 |
A process of purifying G-CSF (Granulocyte Colony Stimulating Factor) in a purification sequence employing chromatography characterized in that
- at least one chromatography is performed using a multimodal resin containing a negatively charged 2-(benzoylamino) butanoic acid ligand
- G-CSF binds to the multimodal resin at a pH between 4 to 6.2, and
- the G-CSF is eluting at a pH 5.5 to 6.5 and wherein the elution is performed with arginine having a concentration in the range of from 0.1 M to 2.0 M,
- optionally in combination with an affinity ligand chromatography step employing a yeast derived F ab fragment directed towards the G-CSF. |
