| 摘要 |
The present invention provides a method of producing a protein multimer-nucleic acid complex comprising a protein multimer and any one target component of the protein multimer, which comprises the following steps:
i) translating a nucleic acid encoding the target component into the target component by an in vitro translation system to give a translation product containing a target component-nucleic acid complex containing the target component and the nucleic acid encoding the target component, and
ii) translating a nucleic acid encoding a non-target component constituting the protein multimer together with the target component into the non-target component by adding the nucleic acid encoding the non-target component to the translation product obtained in i) to give the non-target component, associating the non-target component with the target component contained in the target component-nucleic acid complex to form a protein multimer, thus affording the protein multimer-nucleic acid complex comprising the protein multimer and the nucleic acid encoding the target component. By performing in vitro selection such as ribosome display, mRNA display and the like using the present invention, the function such as binding affinity and the like of a complicated protein multimer such as antibody and the like can be increased or improved efficiently. |
