ENHANCED LIGATION REACTIONS

摘要

In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of at least one agent that generates a ligatable terminal 5′ phosphate group by removing an adenylate group from a terminal 5′ phosphate of a nucleic acid. In some embodiments, an aprataxin enzyme can catalyze removal of an adenylate group from a terminal 5′ phosphate of a nucleic acid. In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of an aprataxin enzyme under conditions suitable for ligating nucleic acid ends.

主权项

1. A system for ligating nucleic acids comprising:
(a) a single-stranded polynucleotide template; (b) first and second oligonucleotide probes that abut each other while they are hybridized to the polynucleotide template, thereby forming a nick; (c) a ligase enzyme; and (d) an agent that catalyzes removal of an adenylate group from the terminal 5′ phosphate of nucleic acid.