HEPATOCYTE INDUCTION METHOD

摘要

Provided is a method for inducing hepatocytes from hepatoblasts.　Provided is a method for inducing hepatocytes from hepatoblasts, said method including a step in which hepatoblasts are cultured in a culture medium containing a chemical selected from a group consisting of pregnenolone and an adrenergic agonist. Hepatoblasts can be obtained by culturing endodermal cells in a culture medium containing DMSO, and endodermal cells can be obtained by culturing pluripotent stem cells in a culture medium containing Activin A and a GSK-3β inhibitor. Therefore, by employing the method of the present invention, it is also possible to provide a method of producing hepatocytes from pluripotent stem cells.