| 摘要 |
A probe for detecting a mutation(s) in exon 12 of the NPM1 gene, comprising at least one fluorescently labeled oligonucleotide selected from the group consisting of P5 and P5' below:
(P5) an oligonucleotide comprising a nucleotide sequence complementary to a nucleotide sequence of 12 to 50 consecutive nucleotides containing nucleotides 145 to 156 in SEQ ID NO:2 or a homologous sequence thereof, wherein the nucleotide corresponding to the nucleotide at position 145 is a cytosine labeled with a fluorescent dye; and
(P5') an oligonucleotide comprising a nucleotide sequence complementary to a nucleotide sequence of 12 to 50 consecutive nucleotides containing nucleotides 145 to 156 in SEQ ID NO:2 or a sequence which hybridizes with the nucleotide sequence in SEQ ID NO:2 under stringent conditions, wherein the nucleotide corresponding to the nucleotide at position 145 is a cytosine labeled with a fluorescent dye. |
