COVALENTLY-IMMOBILIZED HYDROGEL ARRAYS IN MULTI-WELL PLATES

摘要

Hydrogel arrays, methods for preparing hydrogel arrays and methods for screening cell-substrate interactions using the hydrogel arrays are disclosed. Advantageously, the hydrogel arrays include individual hydrogel posts that are completely isolatable, allowing for systematic and independent control of the chemical composition and physical dimensions of each hydrogel post.

主权项

1. A method of preparing a hydrogel array comprising a multi-well plate, the multi-well plate comprising a plurality of wells wherein the plurality of wells independently comprises a hydrogel post, the method comprising
thiol-functionalizing a bottom surface of the plurality of wells; adding a hydrogel precursor solution to at least one well of the plurality of wells; selectively polymerizing a portion of the hydrogel precursor solution in the at least one well; and removing unpolymerized hydrogel precursor solution from the plurality of wells, wherein the polymerized hydrogel forms a hydrogel post covalently immobilized within the at least one well.