TWO-DIMENSIONAL GEL ELECTROPHORESIS APPARATUS AND METHOD

摘要

Two-dimensional gel electrophoresis apparatus includes an electrophoresis zone having four edges defined by a plurality of electrodes. A pair of opposed edges are defined by groups of discrete electrodes. Discrete electrodes within each group are electrically isolated from each other while the other pair of opposed edges are used to generate an electrical field. As a result, the electrical field is less distorted than would be the case if each edge was defined by a single elongate electrode. The apparatus can be provided as a cassette with electrodes configured to guide gas generated during electrolysis out of the cassette through apertures, to reduce the build up of combustible gases.

主权项

1. A two-dimensional gel electrophoresis apparatus comprising:
a plurality of electrodes defining an electrophoresis zone; and a gel support for retaining an electrophoresis gel in the electrophoresis zone, the electrophoresis zone having at least four edges, each of which is defined by at least one of the said plurality of electrodes, the at least four edges comprising first and second pairs of opposed edges, wherein at least one of the second pair of edges is defined by a group of discrete electrodes, some or all of the electrodes having an outer wall with a generally concave formation on the side facing the opposed edge of the electrophoresis zone.