PIVOTING PRESSURIZED SINGLE USE BIOREACTOR

摘要

Pressurized hermetically sealed bags disposed inside a cylindrical support and containing a septum with variable density of porosity and dividing the bag into two chambers are used to provide optimal mixing and gasification of nutrient media to grow a variety of biological cultures, particularly the cell cultures to produce a multitude of pharmaceutical and biotechnology products in a disposable system.

主权项

1. A mixing device comprising:
a) a cylindrical support with an inner surface configured to immovably retain a single-use flexible container; b) a single-use flexible container capable of containing a liquid comprising:
i) A flexible septum with an inner surface immovably positioned within the flexible container and defining a right chamber and a left chamber, the septum comprising a plurality of pores with a variable density over the surface of the septum;ii) At least one liquid inlet;iii) At least one liquid outlet;iv) At least one gas inlet in fluid communication with a source of compressed gas;v) At least one gas outlet capable of controlling the rate of flow of gas;vi) at least one sensor to measure the temperature of a liquid in the flexible container; andvii) A heater and/or a cooling element for controlling the temperature of the flexible container; c) a shaft for pivoting the cylindrical support along its circular axis between −110 to +110 degrees;and wherein, the flexible container is immovably fixed to the inner surface of the cylindrical support.