A PROCESS FOR ISOLATION AND PURIFICATION OF A TARGET PROTEIN FREE OF PRION PROTEIN (PRPSC)

摘要

A process for isolation and purification of a target protein by chromatography wherein the chromatography removes or depletes prions (PrP Sc ), comprising the steps of
- contacting a potentially PrP SC -contaminated sample comprising a target protein with a multimodal chromatographic material;
- setting buffer conditions so that the target protein is bound to the multimodal chromatographic material and whereas PrP SC is not binding to the multimodal chromatographic material;
- followed by elution of the target protein.