反応特異性の増加のための非標準塩基を含む増幅プライマー

摘要

Described herein are methods for increasing the annealing specificity of an amplification reaction using Iso-base Amplification Primers ("IAPs"). IAPs containing an iso-region are capable of regulating sequence-specific annealing thereby enhancing primer-template hybridization for sequence-specific amplification of nucleotides.