发明名称 |
METHODS AND COMPOSITIONS FOR CO-EXPRESSION OF POLYPEPTIDES OF INTEREST AND IL6 |
摘要 |
The present invention provides a CHO cell that includes a polynucleotide encoding a polypeptide of interest. The CHO cell may include a heterologous polynucleotide encoding IL6 and/or an IL6 pathway member or, in the absence of the heterologous IL6 or an IL6 pathway member polynucleotide, the CHO cell can be cultured in the presence of exogenously added IL6 polypeptide. Such CHO cells exhibit superior expression of the polypeptide of interest in culture. Methods for expressing a polypeptide of interest and methods for making such CHO cells are part of the present invention. |
申请公布号 |
EP3033415(A1) |
申请公布日期 |
2016.06.22 |
申请号 |
EP20140836140 |
申请日期 |
2014.08.07 |
申请人 |
MERCK SHARP & DOHME CORP. |
发明人 |
CHEN, JANICE;SHI, SHUANGPING |
分类号 |
C12N5/07;C12N5/02;C12N15/00;C12N15/87 |
主分类号 |
C12N5/07 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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