发明名称 |
MATERIALS AND METHODS FOR PRODUCTION OF BI-FUNCTIONAL FATTY ACIDS IN RECOMBINANT BACTERIA |
摘要 |
A method of producing bi-functional fatty acids comprising introducing into a host cell or organism, which comprises one or more ω- or ω-1 functionalized acyl-CoAs, and expressing therein a KASIII, which can use one or more of the ω- or ω-1 functionalized acyl-CoAs as a substrate; a method of producing a ω-1 hydroxy branched fatty acid, a ω-1 branched fatty acid, or a combination thereof by culturing a mutant E. coli, which does not express a functional KASIII from the endogenous fabH gene and expresses a phaA and a phaB and a functional exogenous KASIII; and a mutant E. coli, a method of making the mutant, a culture comprising the mutant, and a composition comprising ω-1 hydroxy branched fatty acids, a ω-1 branched fatty acids, or a combination thereof obtained from the culture. |
申请公布号 |
US2016168603(A1) |
申请公布日期 |
2016.06.16 |
申请号 |
US201514954972 |
申请日期 |
2015.11.30 |
申请人 |
OMEGACHEA BIORENEWABLES LLC ;IOWA STATE UNIVERSITY RESEARCH FOUNDATION, INC. |
发明人 |
Garg Shivani;Yu Xiaochen;Jin Huanan;Yandeau-Nelson Marna;Nikolau Basil;Rizhsky Ludmila |
分类号 |
C12P7/64;C12N9/10;C12N15/70 |
主分类号 |
C12P7/64 |
代理机构 |
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代理人 |
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主权项 |
1. A method of producing bi-functional fatty acids in a host cell or organism, which method comprises introducing into a host cell or organism, which comprises one or more ω- or ω-1 functionalized acyl-CoAs, and expressing therein a nucleic acid molecule comprising a nucleotide sequence encoding a 3-ketoacyl-acyl carrier protein (ACP) synthase III (KASIII), which can use one or more of the ω- or ω-1 functionalized acyl-CoAs as a substrate, whereupon bi-functional fatty acids in the host cell or organism are produced. |
地址 |
Ames IA US |