Plant Produced Human Papillomavirus Pseudovirion

摘要

The present invention relates to a method of producing HPV pseudovirions in plant cells, the plant produced pseudovirions per se, a neutralisation assay using the plant produced pseudovirions and pharmaceutical compositions comprising the plant produced pseudovirions.

主权项

1. A method for producing a human papillomavirus (HPV) pseudovirion in a plant cell, the method comprising the steps of:
(i) introducing into the plant cell:
(a) a first nucleic acid encoding a HPV L1 polypeptide; and(b) a second nucleic acid encoding a HPV L2 polypeptide,wherein the first and second nucleic acids are contained on at least one expression vector,(c) a replicating vector comprising a third nucleic acid encoding a heterologous polypeptide; (ii) expressing the HPV L1 polypeptides and HPV L2 polypeptides in the plant cell, and (iii) replicating the replicating vector in the plant cell, in order to produce a high copy number of the replicating vector in the plant cell, wherein the expressed HPV L1 and HPV L2 polypeptides assemble, together with a copy of the replicating vector and encapsidate the replicating vector to produce a HPV pseudovirion.