METHOD FOR PRODUCING CELL CONCENTRATE, AND CELL SUSPENSION TREATMENT SYSTEM

摘要

In a method for producing a cell concentrate using a cell suspension treatment system including a storage container of a cell suspension, which has a solution inlet port, a circulation outlet port, and a circulation inlet port, a cell suspension treatment device for concentrating the cell suspension by separating liquid from the cell suspension by filtration, the device including a container having a cell suspension introduction port, a cell suspension lead-out port, and a filtrate outlet, which is filled with a hollow fiber separation membrane, a circulation circuit for concentrating the cell suspension while circulating the cell suspension between the storage container and the cell suspension treatment device, a collection container of a cell concentrate obtained by concentration, a collection path for feeding the cell concentrate to the collection container, an injection path for injecting a solution into the solution inlet port of the storage container, and a detecting unit.

主权项

1. A method for producing a cell concentrate using a cell suspension treatment system including:
a storage container of a cell suspension, which has a solution inlet port, a circulation outlet port, and a circulation inlet port; a cell suspension treatment device for concentrating the cell suspension by separating liquid from the cell suspension by filtration, the device including a container having a cell suspension introduction port, a cell suspension lead-out port, and a filtrate outlet, which is filled with a hollow fiber separation membrane, a circulation circuit for concentrating the cell suspension while circulating the cell suspension between the storage container and the cell suspension treatment device, the circuit being constituted by an introduction communication tube communicating the circulation inlet port of the storage container and the cell suspension introduction port of the cell suspension treatment device and a lead-out communication tube communicating the cell suspension lead-out port of the cell suspension treatment device and the circulation outlet port of the storage container; a collection container of a cell concentrate obtained by concentration; a collection path for feeding the cell concentrate in the storage container, the cell suspension treatment device, and the circulation circuit to the collection container; an injection path for injecting a solution into the solution inlet port of the storage container; and a detecting unit for detecting a volume of the cell concentrate in the storage container or the introduction communication tube of the circulation circuit, the method comprising: a) a step of storing the cell suspension in the storage container by supplying the cell suspension to the storage container through the solution inlet port; b) a step of concentrating the cell suspension by feeding the cell suspension in the storage container to the cell suspension treatment device through the introduction communication tube of the circulation circuit, and then, circulating the cell suspension into the storage container through the lead-out communication tube of the circulation circuit; c) a step of terminating the step b) by detecting the volume of the cell concentrate in the storage container or the introduction communication tube of the circulation circuit; and d) a step of feeding and collecting the cell concentrate in the storage container, the cell suspension treatment device, and the circulation circuit to the collection container through the collection path.