发明名称 |
METHOD FOR MANUFACTURING MULTILAYERED CELL SHEET, MULTILAYERED CELL SHEET HAVING VASCULAR NETWORK OBTAINED THEREBY, METHOD OF USE THEREOF |
摘要 |
A method for manufacturing a multilayered cell sheet characterized in: fabricating a vascular bed that constructs a vascular network extending to the surface from a channel for perfusing a medium, the channel being embedded in a gel; and layering a cell sheet onto the vascular bed to construct a vascular network in the cell sheet. This manufacturing method makes it possible to construct a vascular network in the cell sheet and to fabricate a thick multilayered cell sheet in a simple manner by layering cell sheets. Such a thick multilayered cell sheet is useful as an in-vivo tissue substitute in regenerative medicine involving a variety of tissues. |
申请公布号 |
EP2617811(B1) |
申请公布日期 |
2016.06.15 |
申请号 |
EP20110825225 |
申请日期 |
2011.09.14 |
申请人 |
TOKYO WOMEN'S MEDICAL UNIVERSITY |
发明人 |
SAKAGUCHI, KATSUHISA;SHIMIZU, TATSUYA;SEKINE, HIDEKAZU;UMEZU, MITSUO;OKANO, TERUO |
分类号 |
C12N5/071;A61K35/12;A61K35/34;A61P9/00;A61P9/04;A61P9/10;C12M3/04 |
主分类号 |
C12N5/071 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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