| 摘要 |
A method for biologically producing methanol is disclosed. In some embodiments, the method includes the following: providing a biomass including ammonia oxidizing bacteria having ammonia monooxygenase enzymes and hydroxylamine oxidoreductase enzymes; feeding ammonia to the ammonia oxidizing bacteria; feeding a non-substrate-organic compound including methane to the ammonia oxidizing bacteria; feeding oxygen and reducing equivalents (in the form of hydroxylamine) to the ammonia oxidizing bacteria; oxidizing the ammonia using the ammonia monooxygenase enzymes in the ammonia oxidizing bacteria to generate hydroxylamine and oxidizing the hydroxylamine using the hydroxylamine oxidoreductase enzymes to form nitrite; and partially oxidizing the methane in the compound using the ammonia monooxygenase enzymes in the ammonia oxidizing bacteria to generate methanol. |
