一种稳定性好、准确度高的α-羟丁酸脱氢酶检测试剂

摘要

本发明涉及α-羟丁酸脱氢酶检测技术领域，其中试剂R1中包含缓冲液、BSA、2-酮丁酸、EMULGEN-707、羟基乙叉二膦酸、十八烷基三甲基溴化铵、烷基酚聚氧乙烯醚(APEO）、抗坏血酸氧化酶、胆红素氧化酶、蔗糖、海藻糖、黄原胶、防腐剂；试剂R2的组分为：缓冲液、NADH黄原胶、防腐剂。在试剂R1中加入了羟基乙叉二膦酸，能够有效的螯合重金属离子，并且能够较好的提高试剂的准确性；本发明还在试剂中加入了抗化学酸氧化酶和胆红素氧化酶，能去除抗坏血酸和胆红素的干扰。添加稳定剂BSA、蔗糖、海藻糖、黄原胶，增强试剂稳定性；采用新型表面活性剂烷基酚聚氧乙烯醚(APEO)，显著改善测定的性能，且增强试剂的稳定性。

主权项

一种稳定性更好、准确度高的α‑羟丁酸脱氢酶检测试剂，其特征在于包括试剂R1和试剂R2，所述试剂R1和试剂R2的组成如下：1）试剂R1中含有缓冲液_{····················································}100mmol/L，BSA_{·······················································}1g/L2‑酮丁酸_{··················································}4.4mmol/L，EMULGEN‑707_{···············································}0.7ml/L羟基乙叉二膦酸_{············································}1ml/L‑5 ml/L十八烷基三甲基溴化铵_{······································}0.1g/L‑1g/L烷基酚聚氧乙烯醚( APEO)_{···································}2g/L抗坏血酸氧化酶_{············································}1KU/L‑5 KU/L，胆红素氧化酶_{··············································}1KU/L‑3 KU/L，蔗糖_{······················································}5g/L海藻糖_{····················································}5g/L黄原胶_{····················································}3.8g/L，防腐剂_{····················································}0.5ml/L‑1 ml/L；2）试剂R2的组分为：缓冲液_{····················································}100mmol/L，NADH_{······················································}4.4mmol/L，黄原胶_{····················································}3.8g/L，防腐剂_{····················································}0.5ml/L‑1 ml/L。