发明名称 |
MICROFLUIDIC DEVICES AND SYSTEMS |
摘要 |
We describe a method of label-free cell and/or particle sorting in a microfluidic device, the method comprising; providing a stream of aqueous droplets in oil in a channel of said microfluidic device, wherein at least some of said droplets include cells and/or particles illuminating said stream from a first direction; detecting, for example, scattered light from cells and/or particles within said aqueous droplets in a second direction; determining, for example, a number of said cells and/or particles in each droplet from the scattered light; and sorting said aqueous droplets into one of a plurality of differentiated streams responsive to said determined number. The method further comprises modifying a refractive index of said oil to more closely match a refractive index of said aqueous droplets to reduce light scattered from boundaries of said droplets. Optionally a volume of the droplets is also controlled. In embodiments the oil is fluorous oil. We describe examples of preferred refractive index modifying compounds. |
申请公布号 |
EP3027322(A2) |
申请公布日期 |
2016.06.08 |
申请号 |
EP20140749966 |
申请日期 |
2014.07.30 |
申请人 |
SPHERE FLUIDICS LIMITED |
发明人 |
SMITH, CLIVE A.;LI, XIN;ENESA, KARINE;LIU, XIN;CRAIG, FRANK F.;WHYTE, GRAEME |
分类号 |
B01L3/00 |
主分类号 |
B01L3/00 |
代理机构 |
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代理人 |
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