LIGAND FUNCTIONAL SUBSTRATES

摘要

A substrate comprising a crosslinked polymer primer layer, and grafted thereto a ligand-functionalized polymer is provided. The grafted polymer has the requisite affinity for binding neutral or negatively charged biomaterials, such as cells, cell debris, bacteria, spores, viruses, nucleic acids, and proteins, at pH's near or below the pI's of the biomaterials.

主权项

1. A method of separating a target biological species from a biological fluid comprising contacting the fluid with a ligand-functionalized substrate comprising:
a) a substrate b) a primer layer disposed on the substrate comprising:
a crosslinked polyamine polymer having ethylenically unsaturated polymerizable groups; and c) a ligand-functional alkenyl (co)polymer layer grafted to the ethylenically unsaturated polymerizable group of the primer layer;
whereby a complex comprising the functionalized substrate and the target biological species is formed, and separating the complex; wherein said target biological species is selected from biomacromolecules and microbiological species.