METHOD FOR PREPARING BACTERIAL POLYSACCHARIDE-MODIFIED RECOMBINANT FUSION PROTEIN AND USE THEREOF

摘要

Provided are a method for preparing a bacterial polysaccharide-modified recombinant fusion protein and a use thereof. The method comprises: the recombinant fusion protein and the neisseria meningitidis O-oligosaccharyltransferase PgIL are co-expressed in a bacteria defective in O-antigen ligase genes, and the polysaccharides which are endogenous or exogenous for the bacteria itself are connected to the recombinant fusion protein by the O-oligosaccharyltransferase PgIL, and the polysaccharide-modified recombinant fusion protein is thus obtained. The protein can be used for preparing antibodies and vaccines against bacterial polysaccharides.