PREPARATION OF BEE VENOM WITH ALLERGENIC COMPONENTS REMOVED

摘要

Disclosed is a method for manufacturing a separated and purified bee venom (separated PBV) not possessing allergy-triggering effects by reducing or eliminating allergy-inducing components contained in a bee venom via ultrafiltration, thereby producing a PVB with desirable pharmacological effects while fundamentally blocking allergies that may be induced by the same. The manufacturing method of the present invention includes a first step of preparing a purified bee venom powder; a second step of preparing a bee venom liquid by mixing the purified bee venom powder with distilled water; a third step of removing allergic ingredients contained in the bee venom liquid via ultrafiltration through a membrane having a cut-off size of 10 kDa; and a fourth step of obtaining a separated PBV with the allergenic components removed; wherein the distilled water to be mixed with the purified bee venom powder in the second step is used in the range of from 1000 mL to 1500 mL per 1 g of the purified bee venom powder, the allergenic components removed through the ultrafiltration membrane in the third step are phospholipase A2 and hyaluronidase, and the separated PBV obtained in the fourth step contains 4 wt % or higher of apamin, and 50 wt % or higher of melittin.

主权项

1. A method for manufacturing a separated and purified bee venom (separated PBV), comprising:
a first step of preparing a purified bee venom powder; a second step of preparing a bee venom liquid by mixing the purified bee venom powder with distilled water; a third step of removing allergenic components contained in the bee venom liquid via ultrafiltration through a membrane having a cut-off size of 10 kDa; and a fourth step of obtaining a separated PBV with allergenic components removed; wherein the distilled water to be mixed with the purified bee venom powder in the second step is used in the range of from 1000 mL to 1500 mL per 1 g of the purified bee venom powder; the allergenic components removed through the ultrafiltration membrane in the third step are phospholipase A2 and hyaluronidase; and the separated PBV obtained in the fourth step comprises 4 wt % or higher of apamin, and 50 wt % or higher of melittin.