发明名称 |
Absolute Quantitation of Proteins and Protein Modifications by Mass Spectrometry with Multiplexed Internal Standards |
摘要 |
A method for absolute protein or peptide quantitation by mass spectroscopy. A sample containing a protein or peptide of interest is prepared for mass spectroscopy analysis. The sample is subjected to mass spectroscopy analysis at low resolution whereby a single additive mass spectroscopy peak is obtained, then is subjected to high resolution mass spectroscopy analysis whereby a plurality of mass spectroscopy peaks are obtained. The intensity of each of the plurality of mass spectroscopy peaks is quantitated either by comparison to an internal standard set, or by using a standard curve generated for each isotopologue set. Quantitation using a standard curve enhances quantitation across a dynamic range of analyte. |
申请公布号 |
US2016154006(A1) |
申请公布日期 |
2016.06.02 |
申请号 |
US201514980155 |
申请日期 |
2015.12.28 |
申请人 |
Pierce Biotechnology, Inc. |
发明人 |
Hermanson Greg;Rogers John Charles;Louette Joel;Bomgarden Ryan Daniel;Patel Bhavinkumar |
分类号 |
G01N33/68;H01J49/26;H01J49/00 |
主分类号 |
G01N33/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
Rockford IL US |