ANTIGEN-BINDING PROTEINS SPECIFIC FOR HLA-A2-RESTRICTED WILMS TUMOR 1 PEPTIDE

摘要

Antigen-binding proteins specific for HLA-A2-restricted Wilms tumor 1 peptide are disclosed. The antigen-binding proteins encompass antibodies in a variety of forms, including full-length antibodies, substantially intact antibodies, Fab fragments, F(ab′)2 fragments, and single chain Fv (scFv) fragments, as well as chimeric antigen receptors. Fusion proteins, such as scFv fusions with immunoglobulin or T-cell receptor domains, incorporating the antigen-binding proteins are provided. Methods of using the antigen-binding proteins in the treatment of hyperproliferative diseases such as cancer are also disclosed.

主权项

1. An isolated antigen-binding protein or fragment or derivative thereof comprising one of: (A) an antigen-binding region comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 2 to 8 or 27; or (B) an antigen-binding region comprising a heavy chain variable domain (VH) and a light chain variable domain (VL), wherein the VH and VL, respectively, comprise amino acid sequences selected from the group consisting of SEQ ID NOs: (i) 9 and 13; (ii) 9 and 15; (iii) 11 and 16; (iv) 9 and 17; (v) 12 and 14; (vi) 9 and 14; and (vii) 10 and 13; or (C) an antigen-binding region comprising: (i) the following three heavy chain (HC) CDRs: (a) a HC CDR1 comprising the amino acid sequence set forth in SEQ ID NO: 18; and (b) a HC CDR2 comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 19 and 20; and (c) a HC CDR3 comprising the amino acid sequence set forth in SEQ ID NO: 21; and (ii) the following three light chain (LC) complementarity determining regions (CDRs): (a) a LC CDR1 comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 22 and 23; and (b) a LC CDR2 comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 24 and 25; and (c) a LC CDR3 comprising the amino acid sequence set forth in SEQ ID NO: 26.