ANALYTIC SUBSTRATE COATING APPARATUS AND METHOD

摘要

An apparatus and method for producing a coated analytic substrate using a compact and portable automated instrument located in the laboratory setting at the point of use which can consistently produce one or a plurality of coated analytic substrates “on demand” for using the analytic substrate immediately after coating, preferably without a step of rinsing the coated analytic substrate before use. The apparatus preferably uses applicator cartridges having a reservoir containing the coating compositions used to form the coatings. Preferably the cartridges are removable and interchangeable to facilitate the production of individual analytic substrates having different coatings or different coating patterns. These coated analytic substrates have superior specimen adhesion characteristics due to the improved quality of the coatings applied by the coating apparatus and due to the quickness with which the coated analytic substrates can be used in the lab after production.

主权项

1. A method of applying a paraffin-embedded biological specimen to an analytic substrate, comprising:
wiping a coating composition on an analytic substrate to form a coated surface on at least a portion of the analytic substrate, wherein the coating composition comprises an organofunctional silane; and applying a paraffin-embedded biological specimen to the coated surface of the analytic substrate in a histologic floatation water bath in a way that a hydrolytic area is produced between the paraffin-embedded biological specimen and the analytic substrate wherein in situ hydrolysis of the coating composition is initiated by water from the histologic floatation water bath to produce an alcohol thereby causing a flattening of the paraffin-embedded biological specimen upon the coated surface by removal of water trapped underneath the paraffin-embedded biological specimen and to cause the paraffin-embedded biological specimen to bind to the analytic substrate.