PROTEIN IN WHICH ELECTRICAL INTERACTION IS INTRODUCED WITHIN HYDROPHOBIC INTERACTION SITE AND PREPARATION METHOD THEREFOR

摘要

The present invention provides a protein or antibody in which, of a pair of hydrophobic amino acids selected from within a hydrophobic interaction site of the protein, one hydrophobic amino acid is transformed into a substance having a positive electrical charge and the other hydrophobic amino acid is transformed into a substance having a negative electrical charge, and electrostatic interaction is introduced within the hydrophobic interaction site of the protein by means of the positive charge and the negative charge. The present invention also provides a method for preparing the protein or antibody, and a method for measuring the degree of coupling between a heavy chain and a light chain, using the antibody. The protein or antibody in accordance with to the present invention has a low contamination by a homodimer or a monomer, and thus a heterodimer can be obtained in high purity.

主权项

1. A protein introduced an electrostatic interaction in a hydrophobic interaction site wherein the electrostatic interaction is made between a positive charge and a negative charge, and the positive charge is introduced by a modification from one hydrophobic amino acid to positive charged material in a pair of amino acids in a site of hydrophobic interaction selected from the sites of hydrophobic interaction of protein, and the negative charge is introduced by an modification from the other hydrophobic amino acid to negative charged material in the pair of amino acids.