METHODS FOR IDENTIFYING IMMUNOBINDERS OF CELL-SURFACE ANTIGENS

摘要

The invention provides methods for identifying immunobinders, such as scFv antibodies, capable of specifically binding to cell surface antigens, and compositions identified according to said methods.

主权项

1. A humanized immunobinder capable of binding to an antigen of interest, wherein the immunobinder comprises a variable of binding to an antigen of interest, wherein the immunobinder comprises a variable heavy chain framework sequence having at least 90% identity to the sequence of SEQ ID No. 4, a variable light chain framework sequence, and CDR sequences of immunobinder derived from a B-cell clone identified by:
(a) isolating B-cells from an animal; (b) contacting the B-cells with antigen-expressing cells expressing the antigen of interest or an artificial cell-like body displaying the antigen of interest; and, (c) isolating one or more complexes in which a B-cell is binding to an antigen-expressing cell or said artificial cell-like body displaying the antigen of interest, wherein the isolating is accomplished using a cell sorter, and wherein the B-cell in an isolated complex is identified as a B-cell clone that binds to the antigen of interest.