METHODS AND COMPOSITIONS FOR IDENTIFYING VERTICILLIUM WILT RESISTANT SUNFLOWER PLANTS

摘要

The present invention relates to methods for identifying sunflower lines having increased resistance to Verticillium wilt, and identification of genetic markers linked to gene(s) conditioning such increased disease resistance. The present invention also relates to methods of breeding sunflower plants from lines having increased Verticillium wilt resistance by marker-assisted selection, compositions including nucleic acid probes or primers which are useful for such marker assisted selection, and plants and plant parts produced by such methods.

主权项

1. A method of obtaining sunflower germplasm comprising the steps of: a) assaying sunflower plants for the presence of at least a first genetic marker genetically linked to a QTL that confers resistance to Verticillium wilt; and b) selecting at least a first sunflower plant comprising the genetic marker and the QTL that confers resistance to Verticillium wilt; wherein the QTL maps to a position between the sequence represented by SNP marker DHAG001106 and SNP marker DHAI006271, which map to approximately 0 cM and 54.7 cM on the genetic map of the linkage group termed sunflower chromosome 10; wherein the QTL maps to a position between the sequence represented by SNP marker DHAI005760 and SNP marker DHAI006271 which map to approximately 46.5 cM and 54.7 cM on the genetic map of the linkage group termed sunflower chromosome 10; wherein the QTL maps to a position between the sequence represented by SNP marker DHAI000041 and SNP marker DHAI006271 which map to approximately 47.2 cM and 54.7 cM on the genetic map of the linkage group termed sunflower chromosome 10; wherein the QTL maps to a position tightly linked to the sequence represented by SNP marker DHAG000460 which maps to approximately 8.8 cM on the genetic map of the linkage group termed sunflower chromosome 10, or wherein the QTL maps to a position tightly linked to the sequence represented by SNP marker DHAI007648 which maps to approximately 85.4 cM on the genetic map of the linkage group termed sunflower chromosome 11.