| 主权项 |
1. An automated integrated system for automatically determining concentration of at least one target compound in a sample solution containing one or more non target compounds comprising;
integrated microchip, having,
capillary microchannel affinity column,capillary microchannel separation column,at least two sample solution reservoirs,eluting buffer reservoir,channels interconnecting the reservoirs, the microchannel affinity column and the capillary microchannel separation column, the capillary microchannel affinity column having a surface with immobilizing sites for target compounds, so that as sample solution is passed through the column target compounds are immobilized on the surface, allowing nontarget compounds to remain in solution as the solution is conveyed through and out from the column, and as solution from the eluting buffer reservoir is passed through the capillary microchannel affinity column immobilization of target compounds is reversed to produce target solution containing target compounds; conveying system for selectively applying a driving force to pass solution through the channels from any one of the at least two sample solution reservoirs and eluting buffer reservoir through the capillary microchannel affinity column and for selectively applying a driving force to pass target solution from the capillary microchannel affinity column through the capillary microchannel separation column detection system coupled with the capillary microchannel separation column to produce quantitative measure of concentration of target compounds in target solution passing through capillary microchannel separation column automated system coupled with the conveying systems and detection system for automatic signaling to the conveying system selective application of the driving forces and for automated quantification by comparing quantitative measures of concentration of target compounds from the detection system for multiple target solutions. |
