PERIPHERAL BLOOD STEM CELLS WITH IMPROVED ANGIOGENIC PROPERTIES AND USE THEREOF

摘要

The present application discloses a method for producing peripheral blood stem cells with improved angiogenic properties through EPO or APS priming, and a use thereof. The cells produced according to the method of the present application induce angiogenesis, and thus can be effectively used for the regeneration of, or recovery from, various diseases requiring facilitation of angiogenesis, such as ischemic diseases occurring in the muscle, brain, heart, kidney, or large intestine, e.g. tissue damage, cerebral infarction, stroke, reperfusion injury, myocardial infarction, congestive heart failure, peripheral vascular obstruction, cardiac hypertrophy, low heart contraction, low heart dilatation, maladaptive cardiomegaly, systolic heart failure, diastolic heart failure, hypertensive heart failure, artery or mitral valve diseases, heart blood vessels with pulmonary valve diseases, or ischemic heart blood vessels, caused by ischemia.

主权项

1. A method of producing peripheral blood stem cells (mobPBSC), the method comprising:
collecting peripheral blood from a subject who has been pre-treated with a cytokine; collecting mononuclear cells from the peripheral blood; and treating at least part of the mononuclear cells with an erythropoietin or an activated platelet supernatant (APS) to provide peripheral blood stem cells (mobPBSC).