METHODS AND COMPOSITIONS FOR LONG TERM HEMATOPOIETIC REPOPULATION

摘要

Methods for isolating a CD133+/CD45neg/GlyAneg subpopulation of umbilical cord blood cells are disclosed. In some embodiments, the methods include providing an initial population of umbilical cord blood cells; contacting the initial population of cells with a first antibody that is specific for CD133, a second antibody that is specific for CD45, and a third antibody that is specific for Glycophorin A (GlyA) under conditions sufficient to allow binding of each antibody to its target, if present, on each cell of the initial population of cells; and isolating a subpopulation of cells that are CD133+, CD45neg, and GlyAneg. Also provided are isolated populations of CD133+/GlyAneg/CD45neg stem cells isolated from cord blood, methods for repopulating cell types in subjects, methods for bone marrow transplantation, methods for inducing hematopoietic competency in CD133+/GlyAneg/CD45neg stem cells, and cell culture systems that include CD133+/GlyAneg/CD45neg stem cells.

主权项

1. A method for repopulating a cell type in a subject, the method comprising administering to the subject a composition comprising a plurality of isolated CD133+/GlyAneg/CD45neg stem cells in a pharmaceutically acceptable carrier in an amount and via a route sufficient to allow at least a fraction of the CD133+/GlyAneg/CD45neg stem cells to engraft a target site and differentiate therein, whereby a cell type is repopulated in the subject.