DNA polymerases with increased 3′-mismatch discrimination

摘要

Disclosed are mutant DNA polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.

主权项

1. A recombinant nucleic acid encoding a DNA polymerase having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, or SEQ ID NO:7, wherein the DNA polymerase comprises a motif in the polymerase domain comprising V-D-P-L-M-R-R-A-X6-K-T-V-N-F, and wherein X6 is any amino acid other than A or G (SEQ ID NO:10).