| 摘要 |
A method and system for characterizing cell motion comprising: receiving image data corresponding to a set of images of a cell culture captured at a set of time points; segmenting, from at least one image of the set of images, a cell subpopulation from the cell culture; determining a resting signal for the cell subpopulation; determining a single-peak motion signal based upon the set of images, the set of time points, and the resting signal; detrending the single-peak motion signal of the cell subpopulation based upon the resting signal; determining values of a set of motion features of the cell subpopulation, thereby characterizing cell motion; and clustering the cell subpopulation with at least one other cell subpopulation based upon at least one of the single-peak motion signal and a value of the set of motion features. |
| 主权项 |
1. A method for characterizing cell motion, comprising:
receiving image data corresponding to a set of images of a cardiomyocyte culture captured at a set of time points; performing a time-domain-based determination of a multi-peak motion signal for a cardiomyocyte subpopulation of the cardiomyocyte culture, across a subset of the set of images; generating a comparison between values of the multi-peak motion signal and a threshold condition; determining a resting signal with an associated reference image for the cardiomyocyte subpopulation, based upon the comparison, across a subset of the set of images, wherein determining the resting signal includes identifying a subset of resting-state images from the set of images, wherein a resting-state image has a high similarity to an immediately adjacent image in the set of images; determining a single-peak motion signal based upon the set of images, the set of time points, and the reference image; and determining values of a set of beating features of the cardiomyocyte subpopulation, based upon at least one of the multi-peak motion signal and the single-peak motion signal, and the set of time points, thereby characterizing cell motion, wherein the set of beating features includes at least one of: a beating duration, a beating frequency, and a beating irregularity. |
