Vivo and ex vivo gene transfer into renal tissue using gutless adenovirus vectors

摘要

A method for treating a renal disease in a subject is disclosed. The method includes administering into a kidney of the subject with an effective amount of a gutless adenoviral vector containing a polynucleotide encoding a therapeutic agent. The gutless adenoviral vector contains the nucleotide sequence of SEQ ID NO:13 or SEQ ID NO:15 and expresses the therapeutic agent in a kidney tissue of the subject.

主权项

1. A method for treating a renal disease following evacuation of a clot in a subject, comprising: administering into a kidney vein of said subject an effective amount of a gutless adenoviral vector comprising:
a polynucleotide encoding a therapeutic agent; and a regulatory element operably linked to said polynucleotide, wherein said gutless adenoviral vector comprises the nucleotide sequence of SEQ ID NO: 13 or SEQ ID NO: 15 and expresses said therapeutic agent in a kidney tissue of said subject, and wherein said therapeutic agent is a functional thrombomodulin protein or its variant.